PSG host relegation battling Le Havre on Saturday
Marcelo got a rare start in Tuesday's 2-0 win at home to Getafe which took Real second in the standings and within five points of leaders Atletico Madrid. He produced one of his best performances before hobbling off late in the game.
A statement from Real said the Brazilian had injured his calf without stating how long he would be out for, although Spanish media reports say he will be out for around three weeks.
That rules him out of Real's Champions League last-16 first leg at Atalanta on Feb 24 plus their next three La Liga fixtures at home to Valencia, away to Real Valladolid and at home to Real Sociedad.