New study links deaths of Bangladeshi children to chemicals sprayed on lychee

A new study published in the American Journal of Tropical Medicine and Hygiene has linked the sudden deaths of 13 children in northern Bangladesh in 2012 to chemicals sprayed on lychee fruit trees.

“Excessive and improper” applications of insecticides and other agriculture chemicals in lychee fruit orchards “may have triggered an outbreak of a deadly swelling of the brain known as acute encephalitis syndrome (AES)” that killed those children, according to the study published online.

All of the deaths, which occurred within 20 hours of the onset of symptoms, were linked to exposure to lychee.

The study also pointed to a 2015 outbreak in the same Dinajpur region that involved 12 recorded hospitalisations and 11 deaths as further evidence that the 2012 outbreak is likely associated with the use of toxic chemicals in the area.

The government’s disease monitoring agency, IEDCR, had reported that chemicals sprayed on lychee trees were linked to those deaths.

The new study solidified that evidence.

Scientists also find powerful insecticides, including endosulfan banned in 80 countries, were liberally applied in orchards where many victims played.

The journal states that, as of 2016, Bangladesh was one of several countries, including the United States, that still allowed the restricted use of endosulfan. The pesticide was slated to be phased out of use in the United States by the end of 2016.

However, Director of Plant Protection at the Department of Agricultural Extension Amitav Das told bdnews24.com on Tuesday that they banned the use of endosulfan back in 2014. “Maybe they (researchers) do not have the updates.”

Aggressive use of pesticides is common in Bangladesh due to lack of awareness, scientists say.

There is also a knowledge gap among the farmers about the pesticides withholding period or pre-harvest interval, resulting in pesticide-laced vegetables being supplied to the market.

Agricultural scientists say farmers must wait out a certain period of time before harvesting the crop after spraying pesticides. The time required varies depending on the pesticide group.

“This study makes a strong case for the value of solid detective work and community engagement when investigating the causes of a dangerous and tragic public health crisis,” Patricia F Walker, president of the American Society of Tropical Medicine and Hygiene, said in a statement.

“By working closely with the affected communities and earning their trust, researchers were able to identify the potential role of agricultural chemicals in this outbreak. Community education and improved oversight of pesticide use will be needed to help reduce the risk of future tragedies.”

Similar deaths have been observed near lychee orchards in India; however, a recent analysis published in the Lancet journal concluded that those deaths were caused by a reaction to a naturally occurring toxin found in lychee seeds and pulp.

“Our investigation suggested the seeds might not be the cause as the seeds are not eaten in Bangladesh and the deaths in 2012 were most likely due to an exposure to multiple, highly toxic agrochemicals,” said M Saiful Islam, an associate scientist at International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) and the lead author of the study.

“These deaths occurred at a time when lychee was being harvested and consumed across Bangladesh. If the seeds were the cause, then we would expect to see cases scattered across the country, not just in a certain small area,” he said.

Islam and colleagues from icddr,b, the IEDCR and the United States Centers for Disease Control and Prevention (CDC) based their conclusion on an exhaustive investigation into 14 cases of AES in children 1 to 12 years old that occurred between May 31 and June 30, 2012 in Dinajpur. Only one child survived.

According to the study, 13 of the 14 children lived either right beside or within 10 metres of a lychee orchard.

One victim did not live as close to an orchard, but, before falling ill, he reportedly consumed a large number of lychees collected from the same orchards.

The outbreak occurred around harvest times, when there is typically an abundance of lychee fruit on the ground around the trees.

Local residents told the investigators that it was common for children to play in the orchards and to eat fruit that had fallen on the ground without washing it, using their teeth to peel the lychee’s tough skin.

In addition, several of the victims had family members who worked in the orchards, which, the study notes, could have increased exposures via residues on clothing worn into the home.

Several families of victims reported the symptoms began with a sharp, sudden cry from their child.

Loss of consciousness occurred, on average, about 2.5 hours after the onset of illness and deaths within about 20 hours or less.

Other symptoms included respiratory distress, froth at the mouth and convulsions.

While it is known that an infection like meningitis can lead to AES, the scientists asserted that the “short duration between onset of illness and death all suggest the outbreak was more likely due to a toxic poisoning than an infection.”

Islam said physical evidence collected from the orchards, which included discarded containers of insecticides and other chemicals, and interviews with community residents suggested that “multiple chemicals were applied to the fruit and in amounts far greater than are normally used by other lychee producers”.

The study also found evidence that the lychee growers were applying an insecticide that had been approved only for use in cotton, not food crops.

“People in the communities told us that sometimes the spraying was so heavy it became difficult to stay in their houses and that the smell would linger for hours,” he said.

Islam and his colleagues plan to conduct follow-up studies in an effort to obtain more biological evidence from victims -- specifically liver and brain biopsies along with a focused ethnographic study that could provide more definitive evidence of chemical exposures.

The researchers also noted that clinical symptoms seen in the children were similar to what was noted in an outbreak of sudden child deaths in 2009 in Bangladesh that was linked to the carbamate class of insecticides, which were also used in the lychee orchards.