Traffic on the busy Dhaka-Rajshahi highway came to a halt on Saturday morning as over 1,000 students spilled on it, creating a one-km long traffic gridlock.
A large number of police were deployed on the highway.
The anti-quota demonstrators had blocked the same road for several hours on Thursday to press for their demands.

Last week, the PSC published the 34th BCS preliminary test results based on the quota system prompting thousands of students, who had failed to make the grade, to hit streets in protest across the country demanding.
They say they have not been clear the tests despite securing high marks because those will less marks made the grade owing to quotas some enjoyed.
A group of candidates had blocked the capital’s Shahbagh intersection on Wednesday demanding an end to quotas.
The demonstrations continued on Thursday and spread to several universities in the country. Police broke up the demonstrations in Dhaka on Thursday.
Many Islami Chhatra Shibir activists were allegedly involved in orchestrating violence under guise of protesting the quotas despite the PSC's announcement that the results would be reviewed.
Students alleged the quota system weighed against merit which was unfortunate.
Rajshahi University students blocked the road on Saturday after first announcing they wanted to protest.
The university authority locked the main gate and urged the students not to take part in the protests.
Proctor Tariqul Hasan and the demonstrators had heated arguments.
However, the authorities later opened the main gate and allowed the students to observe their protests for 15 minutes.
The students were on the road until 11:30am.
A large number of police were deployed on the road to avert any untoward incident.