"Test conducted by the medical services on Wednesday morning have revealed that FC Barcelona defender Jeremy Mathieu has injured the soleus in his right foot," Barcelona said on their website.
"...which means he's going to be sidelined for an estimated three weeks."
The 32-year-old joins fellow defenders Gerard Pique and Jordi Alba on the sidelines.
Barcelona, who are currently third in La Liga with 19 points, face Granada on Sunday.