Five people were dead after a Flixbus veered to the right of the busy A9 motorway, local media reports
Transport for London (TfL) shocked Uber last month by deeming it unfit to run a taxi service and refusing to renew its licence, citing the firm's approach to reporting serious criminal offences and background checks on drivers.
Khan, a centre-left politician from the opposition Labour party, has previously criticised Uber and said that instead of hiring "an army of PR experts and an army of lawyers" the firm needed to address the issues raised by TfL.
"What gives me confidence about the TFL decision is the fact that the global chief executive officer for Uber apologised to London," Khan told LBC radio.
"I think that bodes well in relation to the humility which hasn't been shown by Uber London or Uber UK," he said.