A photo of the mural was posted on Banksy's official Instagram account on Monday, where the artist usually claims responsibility for works of art
An inner-city ban went into effect on Wednesday, following a court ruling a day earlier that sided against four businesses offering the service.
The court agreed with the city that the beer bikes disrupt public order and block traffic in narrow streets, with riders shouting and urinating in public.
Amsterdam is well known for its “red light” district with brothels and “coffee shops” selling marijuana, but the millions of annual visitors have long been a nuisance for residents.