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The mishap took place in Laksham Upazila on the Comilla-Noakhali Road around 8:15am Tuesday, said Laksham police OC Monowar Hossain.
A Noakhali bound truck rammed into the autorickshaw leaving the driver of the three-wheeler, two passengers and a pedestrian dead on the spot, said the police officer.
Another autorickshaw passenger died after being taken to the Comilla Medical College and Hospital.
The dead have been identified as autorickshaw driver Jasim Uddin, 30, Rozina Akter, 20, Nasrin Akter, 22, Mohsina Begum, 25, and pedestrian Abul Kalam, 32.